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However, in line with the specificity of CPA, the C-terminal residues of the L585I, L585M, and L585V variants were cleaved in vitro upon exposure to the enzyme for 5 hours at 37 °C, as confirmed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis of the C-terminal peptides (Supplementary Table 1)

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Chemical modifications like acetylation and amidation can sometimes be employed to enhance the stability of such peptides